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Objective To observe the photochemical effect of methylene blue (MB) on the inactivation of E. coli in blood and on biological functions of blood cells. Methods The blood was treated with 15 ?mol/L MB, 40 000 Lux halogen lamp irradiation and 0.35 mmol/l rutin for 10～40 min. The photochemical effect of MB on the inactivation of E.coli in blood was observed. The changes of biologic functions of blood cells were measured by means of RBC membrane binding to poly L lysine, reaction promoted by ATP enzyme and neutrophil phagocytosis tests. Results After treatment for 40 min, the E.coli count in blood decreased by 5 log units. The value of T/E of MB and irradiation of halogen lamp was higher than 1. No obvious changes of the morphology of blood cell were found. The negative electrical charges on membrane of RBC decreased by 11.23%～25.49%. After treatment of the blood for 20～30 min, the activity of Na + K +ATP enzyme of RBC membrane increased by 7.60%～20.07%. When the blood had been treated for 10～20 min, the positive rate of neutrophil phagocytosis enhanced by 9.00%. Conclusion MB photochemical treatment can effectively inactivate E. coli in blood. Under morphology specific condition of effectively inactivated blood bacteria, the shapes and functions of blood cells are not altered, but the RBC adhesion may increase by about 11.23%～25.49%.
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Objective To study the potential role of PKC inhibitor Rottlerin in the treatment of asthma. Methods Balb/c mice were sensitized and challenged with ovalbumin(OVA) protein to construct murine experimental model of asthma. Then the mice were treated by means of injection of Rottlerin or phosphate buffered saline (PBS) into the abdominal cavity. Changes of the total serum IgE, pulmonary eosinophils, cytokines and pulmonary inflammation were investigated. Results Injection of Rottlerin at the dose 0.3 mg/kg into the murine abdominal cavity could inhibit the infiltration of pulmonary eosinophils and pulmonary inflammation and significantly decrease the production of total serum IgE and Th 2 cytokines as well. Conclusion Rottlerin can inhibit murine experimental asthma.
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Objective To ascertain the natural infection rate of hemorrhagic fever with renal syndrome virus (HFRSV) among its hosts and the type of the natural foci for providing some baseline data for the immigrant health and epidemic prevention of the Three-Gorge region. Methods Epidemiological survey on the field was performed including epidemiological data collection, ecology of rodents and pathogen detection. HFRS virus antigen of hosts were detected by the direct immunofluorescent assay (DIFA) technique and determination of HFRSV-RNA by ISH were carried out from HFRSV-Ag-positive animals. Results HFRSV-Ag-positive animals were found in 5 migration areas ie Baitao Town of Fuling Section, Wansheng Village of Fengjie County and Dachang Town of Wushan County. The positive hosts were as follows, Rattus Norvegicus, Apodemus agrarius, Anourusurex squamipes, Mus musculus and Rattus flavipectus. The positive rate of HFRSV in the mice of 5 migration areas were 19.4%, 17.0%, 14.0%, 13.7%, and 8.5% respectively. The results showed that the lung tissues of some hosts in all five migration areas were HFRSV-RNA-positive. Baitao Town and Peishi Town were attributed to mixture type epidemic areas while. Kangle Town, Wansheng Village and Dachang Town were domestic rats type epidemic areas. Conclusion This study shows that the five migration areas are natural epidemic foci of HFRS. It is predicted that maximum risk of HFRS breakout or epidemic may take place after the completion of the San Xia Reservoir(the Three-Gorges Reservoir), which results from rodent moving toward higher land. Therefore, deratization and preventive measures for rat are important in migration areas.
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Objective To observe the germicidal efficacy and stability of the telephone disinfection mat that based on mic-capsule of 2% po tentiated glutaraldehyde in laboratory. Methods The experiment was carried out according to the criteira of disinfection microbiology in labora tory issued by the Ministry of Health of the country in 1999. Results The bactericidal efficiency reached 99.99% for Escherichia coli,St aphylococcus aureus and Candida albicans when soaked in 2% potentiated glu taraldehyde for 3 to 5 min and the HBsAg can be destroyed when soaked for 10 min. Conclusion The JM-high efficiency telephone disinfection mat is of good bactericidal and stable.